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Beckman Coulter
Vi-Cell
Simple to operate:
A
cell sample is loaded into the system (minimum 600uL, but can be
diluted and dilution factored into final result), and the instrument
aspirates the sample, mixes in the trypan blue, loads the sample
into the chamber and records: cell count/mL (equivalent of 100
“grids”), percent viability, and size distribution. It then
performs a clean cycle and repeats for the next sample in the
carousel. Sample processing time is approximately 2.5 minutes per
sample, although there are several user programmable features that
can reduce or increase this time.
Real life application note:
At the end of the 19th Century, Pasteur’s research
showed that alcohol production from glucose was
linked directly to yeast. The fermentation property
of yeast is utilized in the beer, wine, and ethanol
production industries. For optimum yeast
fermentation performance, percentage cell viability and
concentration must be monitored. The
Beckman
Coulter Vi-CELL automates the labor intensive
manual trypan blue viability method. In addition,
the Vi-CELL reports accurate and precise cell concentrations.
The Vi-CELL provides the high resolution
necessary for accurate results on yeast and other small cells.
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Vi-Cell automated
Cell Viability Analysis
The Vi-CELL automates the widely
accepted trypan blue cell exclusion method. Historically, cell
viability determinations were performed manually using a light
microscope and hemacytometer. This technique has major shortcomings
due to subjective determination of cell count, as well as manual,
time consuming steps. In comparison to the manual technique, the
Vi-CELL automatically performs the trypan blue with video imaging of
the flow-through cell. Results are obtained in minutes.
For
confirming viable cell populations. Therefore, both cell
concentration and percentage of viable cells must be determined
accurately and precisely. The new Vi-CELL Series Cell Viability
Analyzers provide an automatic and cost effective means to perform
the trypan blue dye exclusion method. This data is essential to the
decision making process for basic tissue culture cell passage and
maintaining optimum culture conditions in bioreactors. Cell and
tissue culture applications in life science research, including
recombinant protein and biopharmaceutical production, require the
accuracy, precision and automation available only in the Vi-CELL
cell viability analyzer.
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Automation of the standard trypan blue
assay |
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% Viability |
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Total Cell concentration |
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Total Viable cell concentration |
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mean cell size (naughty cells) |
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real time cellular images |
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bioprocess monitoring |
Available immediately !
$ Ordering Information
Ready to buy or learn more
about this instrument?
Please
click here
& fill out our contact page
or contact our knowledgeable sales force at
763-712-8717 / 800-745-2710 for more information.
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