Techne Endurance TC-512 Thermal Cycler

Techne Endurance TC-512 Gradient
Thermal Cycler

Techne TC-512 Thermal Cycler

Fold control:
Each block consists of 8 peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block. This ensures that the TC-512 exhibits the most accurately controlled linear gradient available*.

High performance:
A maximum heating rate of 3°C/Sec and block uniformity of <±0.4°C. With or without a gradient......no compromise!

Versatile block format:
The truly user-friendly interchangeable block system allows blocks to be interchanged in a matter of seconds without the need of any tools (40 x 0.5ml 96 x 0.2 ml, 384¹ well, Insitu Block²).

Intuitive programming:
Easy to use software with free upgrades downloadable from the Techne® website as new functionality is added.

Instrument Software Update:
A new innovation for the TC-512 Thermal Cycler is the ability to update the unit software free of charge.

Technical Considerations:

Block temperatures
To provide thermal cyclers with the high performance and specificity required for the protocols of today, heated block design and program versatility are important features.

Incremental/decremental temperatures
Touchdown DNA protocols are where a higher temperature is required for the initial cycles than later cycles. This ensures that only highly specific product is created (amplified) during the critical initial steps. A decreased, less stringent temperature is then used by gradually decreasing the annealing temperature. The effect is a higher yield and more specific product. Therefore, to eliminate the need for programming lengthy error prone protocols, choose a thermal cycler which can automatically incorporate an incremental decrease/increase in temperature.

Incremental/decremental time
Long template DNA is the amplification of large fragments of DNA (e.g. 40kb lambda, 27kb Genomic). To ensure that the extension is fully complete after each cycle, the extension time is increased with each cycle to compensate for the increased product quantity and decreasing concentrations of reagents. As above with temperature, choose a thermal cycler which can automatically increase/decrease hold time at a given temperature.

Peltier technology
The peltier effect is used in many applications for the reliable cooling of sensitive high-temperature components without the need for refrigerants or compressors. Otherwise known as thermoelectric coolers, peltiers are a special type of semiconductor that function as a solid state heat pump. By applying a low voltage DC power source to one side, heat will be moved in the direction of the current (+ to - ). The heat is thus pumped from one side of the module to the other, so that one face will be cold while the opposite face will be heated. As this effect is reversible, peltier technology is the chosen method for heating and cooling the block in the best thermal cyclers on the market today.

Techne Thermal Cyclers

Techne TC-512 Endurance Gradient
            96 x 0.2 ml block
            60 x 0.5 ml block
Techne TC-412 Endurance
            96 x 0.2 ml block
            60 x 0.5 ml block
Techne TC-312 Endurance
            25 x 0.2 ml block
            20 x 0.5 ml block

When you take a look at these specs, you'll agree they are nothing to sneeze at!

	Better temp ramp rates (adjustable)
	Better block uniformity
	More Peltier (motors) per block
	More T sensors per block
	Heated lid

Wide linear gradient:
The only Gradient Thermal cycler on the market to offer Quad circuit technology which results in the most linear gradient on the market, with an amazing range of 30°C. Protocols can be optimized in a single experiment.

Touch Screen:
The TC-512 incorporates a large, 115 x 90mm, touch-sensitive screen that provides a unique user-friendly programming interface which is second to none.

Graphical display:
The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualization of your experiment’s status. The unique ‘real-time’ graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualization of your experiment’s status.

Memory cards:
Smart Media memory cards provide an alternative to internal memory storage of your programs, offering security and flexibility.

Gradient calculator:
The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.

Don't need a gradient unit ?

Please consider:

Endurance TC-412

Techne TC-412 Endurance
96 x 0.2 ml block
60 x 0.5 ml block

\
Endurance TC-312

Techne TC-312 Endurance
25 x 0.2 ml block
20 x 0.5 ml block

Quotes available at 763-712-8717

Lil Albert says;

"Go Molecular with GMI"

Other Thermal Cyclers:

MJ Research PTC 100 96 well thermal cycler
MJ Research PTC 200 96 well thermal cycler
MJ Research Tetrad thermal cycler
Perkin Elmer PE 9600
Perkin Elmer PE model PE 9700
ABI 7700 Real Time PCR

This figure shows the amplification of samples from a dilution series done with an ABI 7700 real time PCR

Techne TC-312
Techne TC-412
Techne TC-512

TC-512 Specifications
Blocks Formats
0.5ml capacity	60
0.2ml capacity	96
384-well block	Yes
Gradient block	0.5mL, 0.2 mL, 384
In-situ block	Yes
User interchangeable	Yes
Block Temperature Specifications
Gradient range	20°C to 70°C
Maximum gradient	30°C (16°C for 384 block)
Maximum ramp rate*	3.0°C/sec
Block uniformity at 50°C*	<±0.3°C
Column uniformity with a 10°C gradient	<±0.3°C
Temperature range	4°C to 99°C
Temperature set point precision	0.1°C
Heated Lid
Selectable heated lid temperature	100°C - 115°C
Heated lid enable/disable	Yes
Over temperature cut out	Yes
Regulated lid pressure	Yes
Heated lid only comes on if block is set above 35°C
Programming
Memory cards	Yes
Number of programs #	50 or 500 (with card)
Password protection	Yes
Programming	Touchscreen
Graphical display	Real-time graph
Incremental/decremental temperature	Yes
Incremental/decremental hold time	Yes
Maximum hold time	99 hrs
Minimum hold time	1 sec
Variable programmable ramp rate	0.1°C/sec steps
Run end time calculation	Yes
Pause and stop facility	Yes
End of program alarm (enable/disable)	Yes
Auto restart on power failure	Yes
Miscellaneous
Serial port	RS232
Dual Voltage (120/230V)	Yes
Temperature sensor	Thermistor
Peltierelements/block	8
Dimensions L x W x H (mm)	420 x 220 x 260
Footprint L x W (mm)	420 x 220
Connection to PC control program	Yes
Free software upgrades from website	Yes
Power consumption	620 Watts
Frequency	50 - 60 Hz
Net weight	11kg (total)
Warranty	You won't believe it - Call us !
* Given are typical values of a standard 0.2ml block, in an ambient temperature of 20°C.

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