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Randox Evidence Biochip
Array
Features:
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This system
that has the capacity to perform a test output in excess of
2,700 tests per hour* |
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The imaging system that can read multiple analytes simultaneously |
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Biochip technology, a
9mm˛ solid substrate containing an array of discrete test regions
located on the surface of the biochip |
* Based on twenty tests
per chip
Software:
All operational functions of
evidence® are managed and controlled by the dedicated Windows®
based system software. System sensors enable regular updates of
onboard inventory details, which are displayed on the user screen.
The Graphic User Interface (GUI) comprises a main access screen,
three easiview inventory screens and a menu options section.
Easiview screens are used to provide the user with reliable on
screen information on inventory status. The three screens provide
information on the active sample ring, panel-specific reagents and
waste and signal reagents.
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Retrospective
reporting facility to enable
the retrieval of previously unreported tests.
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User-defined cut-off
values for qualitative assays without the need to recalibrate |
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QC graphs and report
function |
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Access to analyser
software for remote maintenance and trouble shooting. |
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The software
restricts access to onboard units and reagent access is also
controlled by the software to ensure safe handling
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Biochips...
....the WAVE of the FUTURE NOW
In the last five years, chip technology has gained
increasing importance in bio- and gene technology. Biochip technology is
characterized by the miniaturization of biochemical and molecular analytical
techniques. With biochips, it is possible to analyze different biological
materials more efficiently and much faster than using conventional
techniques. Generally, this can be done in a more cost-efficient way since
minute amounts of sample are usually sufficient. A biochip the size of a
stamp or fingernail can be used to carry out thousands of biological
reactions in just a few seconds.
Randox has done just that.
The Evidence® system
won the UK’s most prestigious award for innovation in engineering, the
MacRobert award. Presented each year by the Royal Academy of Engineering,
the MacRobert Award recognizes that Evidence®
will make a significant contribution to society, is innovative and will be a
significant commercial success.
The development of biochips is a major thrust of the rapidly growing
biotechnology industry, which encompasses a very diverse range of research
efforts including genomics, proteomics, computational biology, and
pharmaceuticals, among other activities. Advances in these areas are giving
scientists new methods for unraveling the complex biochemical processes
occurring inside cells, with the larger goal of understanding and treating
human diseases. At the same time, the semiconductor industry has been
steadily perfecting the science of microminiaturization. The merging of
these two fields in recent years has enabled biotechnologists to begin
packing their traditionally bulky sensing tools into smaller and smaller
spaces, onto so-called biochips. These chips are essentially miniaturized
laboratories that can perform hundreds or thousands of simultaneous
biochemical reactions. Biochips enable researchers to quickly screen large
numbers of biological analytes for a variety of purposes, from disease
diagnosis to detection of bioterrorism agents.
Microarrays are not limited to DNA analysis; protein
microarrays, antibody microarray, Chemical Compound
Microarray can also be produced using biochips.
Randox Laboratories Ltd. launched Evidence®, the first
protein Biochip Array Technology analyzer in 2003. In
protein Biochip Array Technology, the biochip replaces the
ELISA plate or cuvette as the reaction platform. The biochip
is used to simultaneously analyze a panel of related tests
in a single sample, producing a patient profile. The patient
profile can be used in disease screening, diagnosis,
monitoring disease progression or monitoring treatment.
Performing multiple analyses simultaneously, described as
multiplexing, allows a significant reduction in processing
time and the amount of patient sample required. Biochip
Array Technology is a novel application of a familiar
methodology, using sandwich, competitive and
antibody-capture immunoassays. The difference from
conventional immunoassays is that the capture ligands are
covalently attached to the surface of the biochip in an
ordered array rather than in solution. In sandwich assays
an enzyme-labelled antibody is used; in competitive assays
an enzyme-labelled antigen is used. On antibody-antigen
binding a chemiluminescence reaction produces light.
Detection is by a charge-coupled device (CCD) camera. The
CCD camera is a sensitive and high-resolution sensor able to
accurately detect and quantify very low levels of light. The
test regions are located using a grid pattern then the
chemiluminescence signals are analysed by imaging software
to rapidly and simultaneously quantify the individual
analytes.
Biochip and biosensor technology development today
secures the new advanced level of medicine and pharmacology
of tomorrow. Introduction of new precise and early
diagnostic techniques, turning upside down the current
understanding of untreatable diseases, complex application
of the novel methods of diagnoses and localization, drug
delivery techniques and treatment control will be at a large
extent based on success in development, fabrication and
implementation of novel materials and technologies towards
creating state-of-the-art biochips and biosensors.
You can learn more
about profiting by implementation of BIOCHIP technology in
your laboratory, order reagents
and
instrumentation by calling
763-712-8717 and asking to speak with our biochip sales
specialist or by
completing the form
on the Ordering Page
Need 'conviction' in your
laboratory?....then go for the Evidence!
GMI....instrumental
to the high-tech clinical chemistry laboratory
With over 1000 chemistries available, GMI
together with Randox can provide an instrument system to cover virtually any
test you need to perform in your clinic, hospital or POL:
Randox RX Monza™
Randox RX Daytona™
Randox RX Imola™
Randox Evidence
Investigator™
Randox Evidence Biochip
Array
Randox
Chemistries, Controls, Reagents
Count on GMI and Randox to be your strategic
clinical chemistry and bio-chip partner for instrumentation,
reagents, and reagent rentals.
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Randox
Evidence® Biochip Array
Evidence® the
the best multi-analyte
Biochip Array Technology on the market today. This
integrated analyzer performs simultaneous quantitative detection of
a wide range of protein analytes in a multi-analyte format. The
system may be used for clinical analysis in laboratory medicine,
cutting-edge investigative research laboratories or
in clinical trails. Evidence® performs multiple immunoassays
on each patient sample. The technology platform supports multiple
different protein results, per person, on each biochip.
Introduction
to evidence
Several immunoassay based multi-analyte panels have been developed
for use on evidence®. The assays can be of a competitive or
sandwich format, or in the case of allergens an antibody capture
format.
The method employed is dependent on the molecular weight and the
nature of the analyte. In a competitive assay such as Drugs of
Abuse, the light intensity of the signal produced is inversely
proportional to the concentration of the analyte present due to
competition between the analyte and the conjugated enzyme labelled
derivative for free antibody binding sites.
Alternatively in a sandwich assay such as the cytokine panel the
light signal is directly proportional to the analyte concentration
due to the conjugated enzyme labelled antibody attaching to the
analyte present.
Evidence®
utilizes conventional immunoassay techniques for the measurement of
analytes on the surface of the biochip. The greatest advantage of
this technology is the specific and simultaneous profiling of
biological markers.
After substrate activation, the biochip manufacturing process
requires very accurate and specific placement at discrete test
regions (DTRs) on the biochip surface for the immobilization of the
biological ligand. The number of test sites per biochip is dependant
on the test panel selected and can be up to 100. A chemiluminescent
signal is generated and can be easily detected and quantified by the
unique imaging system.
Evidence® utilizes the basic
principles of immunology; supporting sandwich, competitive and
antibody capture immunoassay formats. However, instead of the
ligands being attached to a microtitre plate, for example in an
ELISA test, the capture antibodies/antigens are attached to the
surface of the biochip.
After addition of a patient sample to the biochip, analytes present
in the sample bind the specific ligands. The degree of binding is
determined using a chemiluminesence light source and quantified
using a Charge-Coupled Detector (CCD) camera and imaging system.
TESTS:
Drugs
of abuse Biochip array
technology
Simultaneously detect 10 drugs classes in a
single patient sample
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Specific test for
10 drugs of abuse |
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Excellent for drug
screening programs |
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Suitable for urine
and whole blood analysis |
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Small sample volume
- measure all 10 analytes using just 7-35 ul of sample
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Fully automated
walk away analyzer and semi-automated analyzer available |
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Excellent
correlation with GC/MS |
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Biochips are
ready-to-use |
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User defined
cut-off limits |
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Barcoded reagents
and samples |
Amphetamine
Methamphetamine
Cocaine
Barbiturates
Cannabinoids
Opiates
Methadone
Benzodiazepines
Phencyclidine
Creatinine
Cytokines
and growth factors array
Interleukin-1a (IL-1a)
Interleukin-1b (IL-1b)
Interleukin-2 (IL-2)
Interleukin-4 (IL-4)
Interleukin-6 (IL-6)
Interleukin-8 (IL-8)
Interleukin-10 (IL-10)
Interleukin-12 (IL-12)*
Vascular endothelial growth factor (VEGF)
Tumor necrosis factor-a (TNF-a)
Interferon-g (IFN-g)
Epidermal growth factor (EGF)
Monocyte chemotactic protein-1 (MCP-1)
* under development
Thyroid array
Free thyroid array
Thyroid stimulating hormone (TSH)
Free tri-idothyronine (FT3)
Free thyroxine (FT4)
Total thyroid array
Thyroid stimulating hormone (TSH)
Total tri-idothyronine (TT3)
Total thyroxine (TT4)
Fertility array
Follicle stimulating hormone (FSH)
Prolactin (PRL)
Lutenising hormone (LH)
Progesterone
Testosterone
Estradiol*
* under development
Tumor monitoring array
Carcinoembryonic antigen (CEA)
a-fetoprotein (AFP)
Human chorionic gonadotrophin (hCG)
Gastrointestinal tumor antigen (CA 19-9)
Ovarian tumor antigen (CA 125)
Breast cancer antigen (CA 15-3)
Tumor PSA array
Carcinoembryonic antigen (CEA)
Total prostate specific antigen (tPSA)
Free prostate specific antigen (fPSA)
Cardiac array
Troponin I
Myoglobin
Creatinine kinase isoform (CK-MB)
Carbonic anhydrase III (CAIII)
Glycogen phosphorylase BB (GPBB)
Fatty acid binding protein (FABP)
Adhesion molecule array
I-CAM
V-CAM
E-selectin
P-selectin
L-selectin
Configuration:
Cassette Loading Tower
Cassette contain vertically stacked biochip carriers in an
easy-load bar-coded cassette. Cassettes are inserted directly and
move downward into a storage compartment
Refrigerated Reagent Storage Unit
The refrigerated reagent storage unit comprises two separate
internal units for panel-specific reagents and chemiluminescent
signal reagents. Bar-coded reagents for the test array enable rapid
identification
Sample Unit
Sample tubes or cups can be continuously loaded onto two removable
sample carousels (180 capacity) each with a barcode reader for rapid
sample identification
Bulk Solutions Unit
Universal wash buffer and displacement fluids are required for
routine operation
Cassette Collection Unit
Cassettes are released into an onboard collection unit when empty or
when the test panel changes
Biohazard Waste Unit
An onboard waste container for used biochip carriers minimizes the
risk of biological contamination and contains an interlocked door
for enhanced safety
You can learn more
about profiting by implementation of BIOCHIP technology in your
laboratory, order reagents and
instrumentation by calling
763-712-8717 and asking to speak with our biochip sales
specialist or by
completing the form
on the Ordering Page
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