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Molecular
Devices Spectra Max
Plus or 250
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Accurate quantitation and superior accuracy |
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Selection of up to 6 wavelengths per assay |
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Uniform microplate temperature control |
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Proprietary PathCheck™ sensor |
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SOFTmax® PRO
software for Windows® and Mac/OS® |
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Incubation & shaking !
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The SPECTRAmax 250 microplate spectrophotometer has a xenon flash
lamp and grating monochromator that facilitate direct UV measurement
of DNA, RNA, and protein in microplates. Up to 96 assays can be run at one time using disposable or quartz
microplates. The purity of DNA can be measured with A260/A280
ratios. Also, users can perform spectral scans from 250 nm to 750 nm
to simplify qualitative analyses of numerous samples. The product
reportedly reduces sample volumes in experiments because the assays
are performed in a microplate instead of cuvettes. SOFTmax PRO
microplate data- analysis software, for use with the SPECTRAmax 250,
provides built-in spreadsheet capabilities for easy data analysis
and reporting
Helpful
information on the specification range for Molecular Devices
Microplate readers
| Emax |
filter based,
400-800nm, 10nm bandwidth,end point ELISA, total protein |
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ThermoMax |
filter based,
400-800nm, 10nm bandwidth, ELISA, total protein + kinetics,
shaking & temperature. This is an excellent
kinetic microplate reader |
| Vmax |
filter based,
400-800nm, 10nm bandwidth, ELISA, total protein + kinetics,
shaking |
| Optimax |
tunable
monochromator, 340-850nm, 5nm bandwidth, temperature control +
shaking |
| SpectraMax 340
Specs: |
tunable
monochromator 340-750nm, 5nm bandwidth, temperature control,
shaking |
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Wavelength Range |
340-850 nm |
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Photometric Range |
0
to 4.000 OD |
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Wavelength Selection |
Monochromator, tunable in 1-nm increments |
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Photometric Linearity (405 nm) |
0
to 3.000 OD |
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Wavelength Bandwidth |
2
nm |
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Photometric Accuracy (340-850 nm) |
<
or = 0.006 OD ± 1.0%, 0-2 OD |
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Wavelength Accuracy |
<±
1.0 nm |
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Photometric Precision |
<
or = 0.003 OD ± 1.0%, 0-2 OD |
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Wavelength Repeatability |
±
0.2 nm |
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Endpoint Read Time |
<
5% compared to a 1 cm cuvette |
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Minimum Kinetic Interval |
96
wells in 9 seconds
384 wells in 29 seconds |
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Temperature Range |
Ambient + 4 °C to 45 °C |
| VersaMax |
Same as
SpectraMax 340 but no scanning capability. 96 well plates
only. |
| SpectraMax 190 |
tunable
monochromator 190-1000nm, 2nm bandwidth temperature control,
shaking, PATHCHECK -
PathCheck sensors measure the depth of the liquid in each
microplate well and normalises the absorbance value to a I cm
pathlenth. Detects pipettor inaccuracies. Calculates
concentration directly |
| SpectraMax Plus |
as above but with
cuvette measuring station |
| SpectraMax Gemini |
Dual scanning
microplate spectrafluorometer |
So, what's an ELISA,
anyway ?
ELISA (Enzyme Linked Immunosorbent Assay)
was first described in 1971, and since then has become a more and
more important technique in diagnostic virology. ELISA has
replaced a number of more cumbersome and time consuming
"classical" serological techniques, and has also widened the scope
of the detection methods for viruses and their related markers of
infection.
When a foreign particle enters the body, the body tries to
synthesize antibodies (defence
particles) , which combine with the antigen making it susceptible
to destruction or phagocytosis.
The same interaction of antigen and antibody outside the body--in
the laboratory--can be used to determine whether a patient has an
infectious or an autoimmune disease. The test measures whether a
specific antibody associated with an illness can be found in a
patient's blood. A positive result indicates that the antibody is
there and implies that the person has encountered a particular
disease.
A portion of serum possibly containing the antibody is allowed to
react with the target antigen. A correct match causes the antigen
and antibody to bind together.
Detection becomes possible when a second antibody is added. This
antibody is prepared from the serum of an animal injected
previously with human antibody; the human antibody in this case
serves as an antigen and the animal thus produces an antibody
against the human antibody. Once isolated, the second antibody can
be chemically linked to a system that can produce a detectable
signal.
In ELISA the label that is added to produce a detectable signal is
an enzyme and therefore it is called enzyme linked immunosorbent
assay.
The basic principles of the ELISA test are as follows:
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Antigens solubilised in an appropriate buffer can be coated on
a plastic surface, like polystyrene. This may be directly or
via an antibody. When serum is added, antibodies can attach to
the antigen on the solid phase.(Antigen-antibody binding)
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The presence or absence of these antibodies can be
demonstrated with the help of anti-human immunoglobulin
conjugate (indirect method) or with conjugate specific against
the appropriate antigen (direct method) respectively. The
antibodies are conjugated to an enzyme, for example
peroxidase.(conjugate binding) |
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Adding a substrate, like HRPO, will detect the amount of bound
conjugate by a degree of colour produced, which can be
quantified. ELISA can also be used for detection of antigens
by using specific antibody on the solid phase. Adding an
enzyme-linked antibody and a substrate leads to colour
production in proportion to the amount of antigen present.
Commercially produced ELISA kits are now available for a wide
range of viral antigens and antibodies, including IgM
antibodies.
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Molecular
Devices Spectra Max
190
The SpectraMax® 190 spectrophotometer is ideal for most life science
applications, especially DNA analysis.The corrected absorbance is within 5% of the
value obtained in a conventional spectrophotometer.
Features:
Use the extinction coefficient of your sample to calculate
concentrations directly.
Correct for volume differences in the
wells.
Expand the dynamic range of your assay.
Detect pipetting errors.
Check performance of your pipetting
devices.
Molecular
Devices Spectra Max 250
250nm to 750nm
The SPECTRAmax instrument's optical design is the only multichannel system
that truly mimics a dual-beam spectrophotometer. Each sample has a
discrete sample beam and reference beam so that each well is measured
directly. The eight-channel system, consisting of 8 sample beams and 8
reference beams, delivers both superior precision and speed of reading
across the microplate. This is a state of the art reader that sold for
approximately $
20,000.00 three years ago. Hurray, this won't last long!
Computer & software, Mac
computer incl.
Excellent condition $
call for quote
$ Ordering Information
Ready to buy or learn more
about this instrument?
Please
click here
& fill out our contact page
or contact our knowledgeable sales force at
763-712-8717 / 800-745-2710 for more information.
Molecular
Devices Spectra Max 340
The SPECTRAmax®
340PC microplate spectrophotometer is the first microplate reader to
incorporate a grating monochromator, allowing the user to tune from
340 to 850 nm without needing to purchase interference filters. If
you need a microplate reader, you need to look at the SPECTRAmax
340PC microplate spectrophotometer.
No Interference Filters
Required
The SPECTRAmax 340PC microplate spectrophotometer does not use
interference filters. Simply select the absorbance maximum of your
sample, and let the monochromator tune to that exact wavelength. Or
scan up to 96 wells at any time to determine the best assay
conditions. The wavelength range is from 340 to 850 nm, tunable in 1
nm increments; scanning range is from 340-850 nm in 1 nm increments.
With the SPECTRAmax 340PC microplate spectrophotometer it's like
having 511 built-in filters, so you will always have the correct
wavelength for any microplate assay.
Accurate Quantitation
The SPECTRAmax instrument's optical design is the only multichannel
system that truly mimics a dual-beam spectrophotometer. Each sample
has a discrete sample beam and reference beam so that each well is
measured directly. The eight-channel system, consisting of 8 sample
beams and 8 reference beams, delivers both superior precision and
speed of reading across the microplate.
Test Accuracy and Precision
of Liquid Handling Devices
The patented PathCheck sensor measures the depth of the liquid in
each microplate well, which information can be used to determine the
volume of liquid dispensed. Volumes as low as 5 µL can be accurately
measured.
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Other Fluorescent Readers:
Thermo
Labsystems
Fluoroskan II fluorescent microplate readers
$ inquire, we have several
in stock
Molecular Devices vMax
Microplate Reader
The Vmax® microplate reader was the first
instrument to provide kinetic reading capability, permitting researchers to
monitor and automatically analyze enzyme kinetics in a microplate.
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Endpoint assays: ELISA (in microplates or
as dot blots), total protein, and quantitation of cytoproliferation by MTT
reduction or by staining with crystal violet |
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IKinetic measurements: enzyme studies,
such as determination of enzymes released from cells (Lactate
dehydrogenase, serine esterase); ELISA |
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Platelet aggregation studies |
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Adapts to your assay design; positive or
negative single- or dual-wavelength kinetics; endpoint assays with speed
read capability |
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AUTOmix ensures adequate mixing before and
between readings of solid phase reactions, such as ELISA assays |
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Onboard microprocessor calculates and
reports the rate of reaction for each well |
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Integrated instrument control and data
analysis with SOFTmax® for your Windows® or Mac OS® computer |
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Measurement range from 400-750 nm; 0.000
to 4.000 OD |
$ Ordering Information
Ready to buy or learn more
about this instrument?
Please
click here
& fill out our contact page
or contact our knowledgeable sales force at
763-712-8717 / 800-745-2710 for more information.
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