Detection method

Fluorescence, Time Resolved Fluorescence, Luminescence: Photomultiplier tube
Absorbance: Silicon photodiode

Read method

Fluorescence and Luminescence: Endpoint, kinetic, well area scanning
Absorbance: Endpoint, kinetic, well area scanning, spectral scanning

Microplate types

6- to 384-well plates
PCR tube trays
Terasaki trays

Reading speed

Fluorescence and Luminescence: 35 sec/96 wells (depends on settings)
Absorbance: 15 sec/96 wells (depends on settings)

Wavelength range

Fluorescence: Excitation 300-650 nm (200-700 nm with TR model),
Emission 300-700 nm (red extended option available)
Time Resolved Fluorescence: Excitation 200-700 nm, Emission 300-700 nm (red extended option available)
Luminescence: 300-700 nm (red extended option available)
Absorbance: 200 to 999 nm, tunable in 1 nm increments

Wavelength selection

Fluorescence: Deep blocking interference filters
Time Resolved Fluorescence: Monochromator
Absorbance: Monochromator

Monochromator wavelength accuracy

+/- 2 nm

Monochromator wavelength repeatability

+/- 0.2 nm

Filter wheel capacity

Fluorescence: 4 excitation positions, 4 emission positions
Absorbance: N/A

Filters supplied

Fluorescence: 4 excitation filters, 4 emission filters
Absorbance: N/A

Bandpass

Fluorescence: Variable, dependent on filter
Time Resolved Fluorescence: 10 nm
Absorbance: 2.4 nm

Dynamic range

Fluorescence: 0 - 99,999 RFU's
Time Resolved Fluorescence: 0 - 99,999 RFU's
Luminescence: 0-99,999 RLU's
Absorbance: 0 - 4.0 OD

OD accuracy

< 1% at 2.0 OD typical
< 3% at 3.0 OD typical

OD linearity

< 1% from 0 to 3.0 OD typical

OD repeatability

< 0.5% at 2.0 OD typical

Resolution

0.001 OD

Sensitivity

Sodium fluorescein 2.5 pg/ml (1.3 fmol/well) typical*
Sodium fluorescein 1 pg/ml (0.5 fmol/well) limit of detection*
Luciferase 0.1 pg/well typical
ATP 100 amol/well limit of detection
(*Black plate, top measurement, 3mm diameter probe)

Standard probes

3 mm top, 5 mm bottom. Top probe height and top or bottom reading position are software adjustable/selectable (fluorescence). Other probe sizes available.

Light source

Fluorescence: Tungsten Halogen 1,000 hrs lifetime
Time Resolved Fluorescence: Xenon flash lamp, 1 billion flashes lifetime
Absorbance: Xenon flash lamp, 1 billion flashes lifetime

Stray light

Absorbance: 0.03% at 230 nm typical

Temperature control

4^oC above ambient to 50^oC

Shaking

Linear shaking: Low, med, high and variable speeds

Reagent dispensers

2 syringe pumps

Dispense volume

5-1000 µl in 1 µl increments

PC Software

KC4 software.
Control through USB or serial port.

Power

100-240 V_AC 50/60 Hz

Dimensions

16"W x 15"D x 10"H
(40.6 x 38 x 25.4 cm)

Weight

40 lbs (18 kg)

Regulatory

For in vitro diagnostic use (Except in the European Union).
All Bio-Tek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact Bio-Tek.