Denaturing Gradient Electrophoresis

Denaturing Gradient Electrophoresis

Major Science
Gradient Mixer, 100ml

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The MV-20-WAVE-DGGE is a complete system for DNA mutation analysis. It is fully equipped with temperature control unit, stirrer, and gradient mixer and casting accessories to perform specific mutation analysis applications. A powerful microprocessor controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70°C, while the simple four screw design of the WAVE insert accelerates set up of denaturing PAGE gels. The flexibility provided by the modular design of the MV-20-WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximizing throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations.

WAVE electrophoresis insert and tank
Max. Number of Gels 2 per run
Plate Dimension (W x H) 20 x 20cm
Active Gel Dimension (W x H) 16 x 17.5cm
Spacer Thicknesses 0.75, 1, 1.5 and 2mm
Max. Sample Capacity 96 samples; 48 per gel
Standard Combs 2 x 1mm 24-sample
Available Combs 1, 5, 10, 18MC, 24, 36MC, 48; as per MV20WAVE and MAXI units
Max. Buffer Volume 8.5L
Unit Dimensions (W x D x H) 40.5 x 17 x 44cm
Weight 8kg
Temperature Control Unit
Temperature Control PID
Operating Temperature Range Ambient - 100ºC
Working Temperature Range (DGGE) 45-70ºC
Buffer Recirculation Mechanism Stirring
Temperature Uniformity / Stability at 37ºC ±0.05/0.02ºC
Setting / Display Resolution 0.1ºC
Safety Fluid-level float switch; isolated; IEC 1010/CE
Stored Temperature Values 4
Heater Power at 230V/110VAC 1.4/1.3kW
Electrical Power at 230V/100VAC 1.5/1.4kW (50-60Hz)
Recommended Power Supply
Voltage 500V
Current 800mA
Power 300W
Gradient Mixer
Total Volume 100ml
Volume of Reservoir & Mixing Chambers 50ml
Internal Diameter of Outlet Port 2mm
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