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Life Tech Attune NxT flow cytometer
Not sure which configuration is right for your application? Schedule a free consultation with our technical consultants today!
Schedule a consultation Have One To Sell?Product Description
- Modular design—Multiple configurations available—this one has them all !
- Simplified sample prep—No wash, no lyse options, non-clogging fluidics
- Enables unique applications—Complex protocols on a broad range of cell types and samples
- Customized 4-laser, 14-color system
- Data at up to 35,000 events/second.
- Detect particles with diameters from 0.5 μm to 50 μm.
The Attune NxT can be designed to accommodate most common fluorophores and fluorescent proteins used in flow cytometry to match most panels you are currently running. Multiple fluorescent proteins can be detected with an (optional) 561 nm laser. The Attune NxT flow cytometer, designed to use acoustic-assisted hydrodynamic focusing, and consequently achieves sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min—up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec. Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations.
Billed as a personal flow cytometer, the Attune NxT AFC2 matches the most sophisticated multi laser multi color instruments on the market today and meets the demands of the most complex experiments.
Technical Specifications
| Lasers | Violet: 405 nM (50 mW) Blue: 488 nM (50 mW) Yellow: 561 nM (50 mW) Red: 637 nM (100 mW) |
| Filters | VL1: 440/50 VL2: 512/25 VL3: 603/48 VL4: 710/50RL1: 670/14 RL2: 720/30 RL3: 780/60YL1: 585/16 YL2: 620/15 YL3: 695/40 YL4: 780/60BL1: 530/30 BL2: 590/40 BL3: 695/40 |
| Scatter | Forward scatter: Photodiode detector with 488/10 Side scatter: PMT with 488/10 |
| Control and Analysis | Dell Desktop with Intel CORE i7 with Windows 7 Attune NxT Software version 2.2 Dell Flat Panel LCD monitor |
| Automated maintenance | startup (less than 15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination |
| Gates | standard and customizable gates |
| Graphics resolution | publication-quality images (customizable) |
| Probe Design | Intelligent probe design minimizes clogging and carryover (less than 1%) – helps prevent damage to the instrument |
| Sample Mixing | Mixes sample by aspiration to maintain sample homogeneity and cell viability |
| Laser | Simmer mode: automatic shutoff prolongs laser usage lifetime by 10xPre-aligned and welded fiber to laser interfacePre-aligned fiber to beam-shaping optics (BSO) interface (alignment-free)All lasers spatially separatedFocus lens per laser: power where you want itFlat-top laser specified at the flow cell Benefits of flat-top lasers |
Replacement Parts & Accessories
To view available parts for this instrument and instruments like it, click here.
Our parts and accessories inventory is constantly changing. Don't see what you're looking for? Give us a call, we'll be happy to help.
Service
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| Preventative Maintenance Visit | 1 Preventative Maintenance Visit per Year | 1 Preventative Maintenance Visit per Year | ||
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