Molecular Devices SpectraMax M2/M2e Microplate Readers
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Multi-detection microplate readers with dual-mode cuvette ports
KEY FEATURES
• Multi-detection capability
• Top- and bottom-read (M2e only)
• Dual-mode cuvette port
• Dual monochromators
• PathCheck and well volume sensor
• Instrument and software validation
• Robot compatible
The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor allow homogeneous and heterogeneous microplate assays to be performed in one flexible system. SpectraMax M2 readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command.
Technical specifications
Absorbance photometric performance
Wavelength selection: Monochromator, tunable in 1 nm increments
Wavelength range: 200–1000 nm
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Wavelength repeatability: ±0.2 nm
Photometric range: 0–4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette): < ±0.005 OD ±1.0%, 0–2 OD
Photometric precision: < ±0.003 OD ±1.0%, 0–2 OD
Baseline flatness: < 0.001 OD
Stray light: < 0.05% @ 230 nm
Fluorescence photometric performance
Dual monochromators: 1 nm increment selection
EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Wavelength bandwidth (EX, EM): 9 nm
Top-read optimized sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed* sensitivity: 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity (M2e only): 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed* sensitivity (M2e only): 25 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
EX Wavelength range: 250–850 nm
EM Wavelength range: M2: 360–850 nm, M2e: 250–850 nm
Data collection: 50–1450 µsec., 200 µsec. increments
Guaranteed sensitivity*: 7 pM Eu-chelate
Luminescence (secondary mode)
Wavelength range (M2): 360–850 nm
Wavelength range (M2e): 250–850 nm
Guaranteed sensitivity*: 50 fM alkaline phosphatase
General photometric performance
Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Read time**
96 wells: Abs 18 sec., FI 15 sec.
384 wells: Abs 49 sec., FI 45 sec.
Shaker time: 0 to 999 seconds
Temperature control: Ambient +4°C to 45°C
Temperature uniformity: < 1°C at 37°C set point
Temperature accuracy: ±1°C at 37°C set point
General specifications
Dimensions (in.): 8.6 (H) x 22.8 (W) x 15 (D)
Dimensions (cm): 22 (H) x 58 (W) x 38 (D)
Weight: 35 lbs. (15.9 kg)
Power consumption: < 125 watts
Power source: 100–240 Vac, 3 A, 50/60 Hz
Robot compatible: Yes
| Weight | 1600 oz |
|---|---|
| Dimensions | 40 × 48 × 48 in |
| SpectraMax Microplate Reader | M2, M2e |
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